Enhancing sprint performance is crucial for football because sprinting is closely linked to key moments before goals, assists and defensive actions. Many studies of strength and sprint performance have compared strength variables to a static start sprint test. However, evidence to date suggests that application of these assessment methods to explain acceleration performance in football may be inappropriate. Recent research has however shown that sprints from a static start or low velocity sprints account for less than 1% of all sprints during a match with sprints from a moving start being the most frequent. This independent action is referred to as pick-up acceleration (PUA) and no studies have been carried out to investigate its underlying strength properties. The primary objective of the study was to assess the relationships between isometric and jump performance factors and PUA performance. The secondary objective was to identify which combination of these variables best explains the differences in PUA performance